1/11/2024 0 Comments Bovi pro tl minitubeCentrifugation (300 x g for 20 min) was done for all methods. Ten unit samples were studied each contained sperm from the cauda epididymides of four cats (total, approximately 200 x 10(6) sperm) and was equally allocated into four treatments: 1) simple washing, 2) single-layer centrifugation through colloid prior to cryopreservation (SLC-PC), 3) single-layer centrifugation through colloid after cryopreservation (SLC-AC), and 4) swim-up. The objectives were to determine the: 1) extent of epithelial and red blood cell contamination in epididymal cat sperm samples recovered by the cutting method 2) efficacy of simple washing, single-layer centrifugation (SLC), and swim-up for selecting epididymal cat sperm and 3) effects of freezing and thawing on cat sperm selected by various techniques. Single-layer centrifugation through colloid selects improved quality of epididymal cat sperm. Despite the high percentage of sperm defects in epididymal cells, regardless of the extender, we concluded that Bovimix(®) is a viable alternative for the freezing of canine epididymal sperm. In contrast, amplitude of lateral head displacement, slow sperm and static sperm were lower in Bovimix(®). The semen parameters, sperm movement index, linearity, total motility and rapid progressive motility were statistically higher in Bovimix(®) than TRIS. The straws were thawed at 56 Â☌ for 10 s and were evaluated for motility by computer assisted analysis (CASA). Samples with ≥ 80% motility were pooled and then divided before dilution and packaging in 0.5 ml plastic straws, equilibration at 4 Â☌ for 1 h, freezing in nitrogen vapour for 20 min and storing at -196 Â☌. The testes of 13 adult dogs were kept at 5 Â☌ for 24 h in saline solution, and epididymal sperm was recovered in Ringers solution without lactate and were evaluated for motility. The objective of this study was to verify the effects of a commercial bovine extender (Bovimix(®) Nutricell, Campinas) and a traditional TRIS-citric acid-glucose-egg yolk-7% glycerol extender on cryopreservation of canine epididymal sperm. The extender type used to freeze sperm is important to avoid spermatozoal membrane damage and to preserve semen quality after cryopreservation. The collection of epididymal sperm is an option for preservation of germplasm of genetically superior animals that need to be orchiectomized or have died. Martins, Mim Justino, R C Sant'anna, M C Trautwein, L G C Souza, F F These results indicate that the disruption of WLS in principal cells of the caput epididymidis inhibits WNT protein secretion but has no effect on sperm motility and male fertility, suggesting that WNT signaling in the caput epididymidis may be dispensable for epididymal sperm maturation in mice.Ĭomparison of two different extenders for cryopreservation of epididymal dog sperm. Moreover, fertility tests showed that Wls cKO male mice had normal fertility. ![]() ![]() Interestingly, sperm motility analysis showed that the WLS deficiency in the caput epididymidis had no effect on sperm motility. In the caput epididymidis of Wls cKO mice, WNT 10A and WNT 2b, which are typically secreted by the principal cells of the caput epididymis, were not secreted. Immunoblot analysis showed that WLS was significantly reduced in the caput epididymidis of Wls cKO mice. In these mice, WNTLESS (WLS), a conserved membrane protein required for all WNT protein secretion, was specifically disrupted in the principal cells of the caput epididymidis. To further investigate WNT signaling function in epididymal sperm maturation, we generated Wntless conditional knockout mice (Wls cKO), Wls flox/flox Lcn5-Cre. ![]() Recently, WNT signaling has been implicated in epididymal sperm maturation. ![]() Despite considerable efforts, the factor(s) promoting epididymal sperm maturation remains unclear. Spermatozoa maturation, a process required for spermatozoa to acquire progressive motility and the ability to fertilize ova, primarily occurs in the caput and corpus of the epididymis. Role of WNT signaling in epididymal sperm maturation.Ĭheng, Jin-Mei Tang, Ji-Xin Li, Jian Wang, Yu-Qian Wang, Xiu-Xia Zhang, Yan Chen, Su-Ren Liu, Yi-Xun
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